Polymerase Chain Reaction

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" The process of PCR occurs in three steps: denaturing, annealing and extension. Each repetition of these steps constitutes one cycle and doubles the amount of DNA present in the sample. Each of these steps is conveniently triggered by temperature. "

​Temperature regulation enables the ingredients for each step to be mixed in the same container. All the researchers need do is mix up the recipe.  The raising and lowering of the temperature does the rest. In denaturing, the temperature is raised to approximately 94°F.

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2. the discovery of pcr:

The discovery of Polymerase Chain Reaction (PCR) technique, by Kary B. Mullis, allowed scientists to generate millions of copies of DNA. In 1991, Roche bought the rights to PCR from Cetus and invested in refining the science for use in molecular diagnostics to detect diseases. Launching the first FDA-approved PCR test in 1993, Roche Molecular Diagnostics has not only defined and refined PCR, but it has remained the clear leader of this technology.

3. how to perform a pCR At lab

bench:

From Biomedical Science Practice, part of the Fundamentals of Biomedical Science series. William Armour is Lecturer in Biomedical Sciences and Laura Towns is a Graduate Assistant at London Metropolitan University

4. funny PCR Song

Scientists for Better PCR - a Bio-Rad Music Video for the OLD school 1000-Series Thermal Cycler! (Grandfather of the MiniPCR that we use at our Biology Lab).

I of IV:  What happens during P.C.R?

II of IV: The discovery

III of IV: how to perform a P.C.R

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IV of IV: PCR Song

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